Differential Nucleolar Staining Affinity with a Modified Papanicolaou Staining Procedure

A modified Papanicolaou staining procedure using diluted Harris' hematoxylin with potassium alum is described. Nucleolar staining varies from blue to bright red. This technique has been applied to mammary tumor cell lines in vitro under several conditions of hormonal stimulation known to induce...

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Veröffentlicht in:	Biotechnic & histochemistry 1981-01, Vol.56 (4), p.215-219
Hauptverfasser:	Mouriquand, Jacqueline, Mouriquand, Claude, Petitpas, Elisabeth, Louis, Josette, Mermet, Marie-Ange
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Nucleolus - drug effects Cell Nucleolus - ultrastructure Dexamethasone - pharmacology Hematoxylin Mammary Neoplasms, Experimental - ultrastructure Mice Prolactin - pharmacology Staining and Labeling - methods
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Zusammenfassung:	A modified Papanicolaou staining procedure using diluted Harris' hematoxylin with potassium alum is described. Nucleolar staining varies from blue to bright red. This technique has been applied to mammary tumor cell lines in vitro under several conditions of hormonal stimulation known to induce protein synthesis and cell differentiation. Blue nucleoli are observed in control resting cells, while bright red nucleoli are seen after hormonal stimulation.
ISSN:	1052-0295 0038-9153 1473-7760
DOI:	10.3109/10520298109067314