A Simple Method to Demonstrate Lipids in Epon-Embedded Ultrathin Sections

Growing oocytes of the marine crab Cancer pagurus L. contain different kinds of yolk inclusions. Some of them are strongly blackened by OsO4 and presumably are lipids. The lipid character of these granules was demonstrated by extracting them from the epon-embedded ultrathin sections by using the met...

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Veröffentlicht in:	Biotechnic & histochemistry 1970-01, Vol.45 (3), p.129-132
Hauptverfasser:	Eurenius, Laila, Jarskär, Rune
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Crustacea - cytology Epoxy Resins Female Lipids - isolation & purification Liver - cytology Methods Mice Microscopy, Electron Microtomy Osmium Ovum - cytology Oxides Solvents Staining and Labeling
Online-Zugang:	Volltext
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Zusammenfassung:	Growing oocytes of the marine crab Cancer pagurus L. contain different kinds of yolk inclusions. Some of them are strongly blackened by OsO4 and presumably are lipids. The lipid character of these granules was demonstrated by extracting them from the epon-embedded ultrathin sections by using the method of Mayor et al. (J. Biophys. Biochem. Cytol., 9:909, 1961) originally recommended for the removal of epoxy resin from sections intended for light microscopy. This technique was also applied to liver tissue of mouse with successful result.
ISSN:	1052-0295 0038-9153 1473-7760
DOI:	10.3109/10520297009085354