The Complete cDNA Sequence Encoding Dog Gastric Lipase:Short Communication

Oligodeoxyribonucleotide ligation to single-stranded cDNA (SLIC) and polymerase chain reaction (PCR) techniques were used to clone an entire dog gastric lipase (DGL) cDNA. The size of the cDNA is confirmed by Northern blot analysis. The DGL is synthesized as a 379-amino acid mature polypeptide with...

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Veröffentlicht in:	DNA sequence 1998, Vol.8 (4), p.257-262
Hauptverfasser:	Vaganay, Sabine, Joliff, Gwennaël, Bertaux, Odile, Toselli, Esther, Devignes, Marie-Dominique, Bénicourt, Claude
Format:	Artikel
Sprache:	eng
Schlagworte:	cloning Fat digestion preduodenal SLIC
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Beschreibung
Zusammenfassung:	Oligodeoxyribonucleotide ligation to single-stranded cDNA (SLIC) and polymerase chain reaction (PCR) techniques were used to clone an entire dog gastric lipase (DGL) cDNA. The size of the cDNA is confirmed by Northern blot analysis. The DGL is synthesized as a 379-amino acid mature polypeptide with a molecular mass of 43176 Da which is preceded by a 19-amino acid signal sequence located at the NH2--terminus. Comparison of the signal sequences reveals a high degree of similitude between the DGL, the human gastric lipase (HGL), the rabbit gastric lipase (RGL) and the rat lingual lipase (RLL).
ISSN:	1042-5179 1029-2365
DOI:	10.3109/10425179809008461