Dialysis cultivation of microorganisms as the appropriate model of population control in ecosystem research

Opportunities for application of methods of ecological control of anthropogenic impact on ecosystems have been considered. The biotic approach based on dialysis cultivation of microorganism populations has been suggested in order to evaluate the ecological state of living systems. Dialysis cultures are characterized with a high level of biomass accumulation and considerable extension of exponential and stationary growth phases. The cell state at dialysis cultivation corresponds to the state of the organisms in native nature conditions. The method for analysis of responses from microbe cells on external factors' influence has been suggested. The method is based on the registration of alterations of time-spatial parameters in cell populations by layer-by-ayer noninvasive analysis using the spectroscopy of internal reflection.