Pathophysiological resarch of autoimmune liver disease: Human cytokine genes expression in SCID mice liver reconstituted with mononuclear cells of patients

Pathophysiological resarch of autoimmune liver disease: Human cytokine genes expression in SCID mice liver reconstituted with mononuclear cells of patients

Peripheral blood mononuclear cells (PBMC) from patients with autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC) and healthy individuals were recostituted into sever combined immunodeficient (SCID) mice to investigate human cytokine genes expression in the mice liver. Production of human imm...

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Veröffentlicht in:Kanzo 1994/07/25, Vol.35(7), pp.518-526
Hauptverfasser: KIMURA, Yasuhiko, Morshed, Syed Ahmed, NISHIOKA, Mikio
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container_title Kanzo
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creator KIMURA, Yasuhiko
Morshed, Syed Ahmed
NISHIOKA, Mikio
description Peripheral blood mononuclear cells (PBMC) from patients with autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC) and healthy individuals were recostituted into sever combined immunodeficient (SCID) mice to investigate human cytokine genes expression in the mice liver. Production of human immunoglobulins (Ig) in SCID mice reconstituted with AIH-PBMC was significantly (p
doi_str_mv 10.2957/kanzo.35.518
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Production of human immunoglobulins (Ig) in SCID mice reconstituted with AIH-PBMC was significantly (p&lt;0.05) increased compared to SCID mice reconstituted with PBC-PBMC and normalPBMC. Infiltrating cells around the portal area were found in all PBMC reconstituted mice, suggesting presence of the graft-versus-host (GVH) reaction. SCID mice liver reconstituted with PBC-PBMC showed severe infiltration with bile duct lesions. Using reverse transcription polymerase chain reaction (RT-PCR), interferon (IFN)-γ gene was detected in all SCID livers, while interleukin (IL)-6 and IL-1β were detected in some SCID livers. IL-6 gene correlated with human Ig levels in SCID sera, whereas increased IL-1β was found in liver with severe bile duct lesions. 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Production of human immunoglobulins (Ig) in SCID mice reconstituted with AIH-PBMC was significantly (p&lt;0.05) increased compared to SCID mice reconstituted with PBC-PBMC and normalPBMC. Infiltrating cells around the portal area were found in all PBMC reconstituted mice, suggesting presence of the graft-versus-host (GVH) reaction. SCID mice liver reconstituted with PBC-PBMC showed severe infiltration with bile duct lesions. Using reverse transcription polymerase chain reaction (RT-PCR), interferon (IFN)-γ gene was detected in all SCID livers, while interleukin (IL)-6 and IL-1β were detected in some SCID livers. IL-6 gene correlated with human Ig levels in SCID sera, whereas increased IL-1β was found in liver with severe bile duct lesions. 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title Pathophysiological resarch of autoimmune liver disease: Human cytokine genes expression in SCID mice liver reconstituted with mononuclear cells of patients
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