A case of multicentric hepatocellular carcinoma diagnosed by flow cytometry

space occupying lesions were indicated by abdominal ultrasonographic examination. Posterior segmentectomy of the liver revealed a yellowish tumor and a greenish tumor. Both of them had thin capsules and were classified as Edmondson-Steiner's grade II. However, the yellowish tumor exhibited remarkable fatty infiltration. The tumor cells and their nuclei were small, and negative for HBs antigen. On the other hand, the greenish tumor showed bile pigmentation over hepatocytes and bile ducts. Flow cytometric determination of the nuclear DNA content of these tumors showed different aneuploid patterns. The DNA index was 1.16 for the yellowish tumor, and 1.28 for the greenish tumor. It was suggested that these nodules occurred multicentrically. Flow cytometric method also would be useful evaluation of multicentric hepatocellular carcinoma.