Assay of Australia Antigen Employing Double-Antibody and Solid-Phase Radioimmunoassay Techniques
Australia antigen (Au) was assayed by double-antibody (RIA-DA), solid-phase (RIA-SP) radioimmunoassay and hemagglutination inhibition (HI) methods. Anti-Au was assayed by passive hemagglutination (PHA). Purified Au was labeled with 131I for RIA-DA. Ausria was used for RIA-SP. RIA-DA was in purified...
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Veröffentlicht in: | Kanzo 1974, Vol.15(7), pp.427-432 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng ; jpn |
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Zusammenfassung: | Australia antigen (Au) was assayed by double-antibody (RIA-DA), solid-phase (RIA-SP) radioimmunoassay and hemagglutination inhibition (HI) methods. Anti-Au was assayed by passive hemagglutination (PHA). Purified Au was labeled with 131I for RIA-DA. Ausria was used for RIA-SP. RIA-DA was in purified Au titers 2- to 4-fold sensitive than RIASP and 10- to 20-fold sensitive than HI. The results in various liver diseases were compared. Sera that were positive only by RIA-SP or that were negative only by HI were found, but there was none which was positive only by RIA-DA. It was suggested that the damage to purified Au might occur when it was labeled with 131I. When Au was assayed by RIA-DA, 24% of the sera which had been positive by PHA showed false-positive. Inhibition studies using normal guinea-pig serum on those found to be positive by RIA-SP indicated that there was none who had antibody to guinea-pig globulin. |
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ISSN: | 0451-4203 1881-3593 |
DOI: | 10.2957/kanzo.15.427 |