Assay of Australia Antigen Employing Double-Antibody and Solid-Phase Radioimmunoassay Techniques

Australia antigen (Au) was assayed by double-antibody (RIA-DA), solid-phase (RIA-SP) radioimmunoassay and hemagglutination inhibition (HI) methods. Anti-Au was assayed by passive hemagglutination (PHA). Purified Au was labeled with 131I for RIA-DA. Ausria was used for RIA-SP. RIA-DA was in purified...

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Veröffentlicht in:Kanzo 1974, Vol.15(7), pp.427-432
Hauptverfasser: KAKUMU, Shinichi, ITO, Shozo, OKUYAMA, Sumihiko, INADA, Seikichi, TAGO, Katsuhiko
Format: Artikel
Sprache:eng ; jpn
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Zusammenfassung:Australia antigen (Au) was assayed by double-antibody (RIA-DA), solid-phase (RIA-SP) radioimmunoassay and hemagglutination inhibition (HI) methods. Anti-Au was assayed by passive hemagglutination (PHA). Purified Au was labeled with 131I for RIA-DA. Ausria was used for RIA-SP. RIA-DA was in purified Au titers 2- to 4-fold sensitive than RIASP and 10- to 20-fold sensitive than HI. The results in various liver diseases were compared. Sera that were positive only by RIA-SP or that were negative only by HI were found, but there was none which was positive only by RIA-DA. It was suggested that the damage to purified Au might occur when it was labeled with 131I. When Au was assayed by RIA-DA, 24% of the sera which had been positive by PHA showed false-positive. Inhibition studies using normal guinea-pig serum on those found to be positive by RIA-SP indicated that there was none who had antibody to guinea-pig globulin.
ISSN:0451-4203
1881-3593
DOI:10.2957/kanzo.15.427