A Novel Efficient MPEG-Chitosan/HA Biopolymer for Adsorption of the Anticancer SN-38 Liquid Dispersions: Kinetics, Thermodynamic and Ex-Vivo Release Evaluation

Amongst several drug delivery schemes for perfect drug delivery comprise biocompatibility, selective aiming of cancer cells, low-cost, and safe process of nanoparticle preparation. In this work, a new mPEG-chitosan/HA biopolymer was prepared as adsorbent nanoparticles (mNPs) for an efficient drug delivery system. The mNPs was synthesized by conjugating poly (ethylene glycol) methyl ether (mPEG) to chitosan and prepared through ionic gelation between mPEG-chitosan and hyaluronic acid (HA). The prepared mNPs were used to adsorption/release of 7-ethyl-10-hydroxycamtothecin (SN-38) from its liquid dispersions. The mNPs adsorbent was characterized by Fourier transforms infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM). The results demonstrated that the adsorption isotherm of SN-38 on mNPs follows Langmuir model, and the adsorption capacity was 346.511 mg g-1. Besides, the pseudo-first order kinetic well fitted the equilibrium data. Further, thermodynamic parameters including ΔH, ΔG and ΔS were calculated which demonstrated that the physical spontaneous adsorption was prevailing. In addition, the ex- vivo release of SN-38 from mNPs were in good agreement with Korsmeyer-Peppas equation indicating the drug release process was governed by diffusion phenomena. The above results revealed that mNPs containing SN-38 was a good candidate for the drug delivery systems. Resumen. Dentro de las diferentes propiedades importantes de los sistemas de liberación de fármacos se encuentran la biocompatibilidad, el ataque selectivo a las células cancerosas, el bajo costo y los procesos adecuados de preparación de nanopartículas. En este trabajo, un nuevo biopolímero de mPEG-chitosan/HA se preparó en la forma de nanopartículas (mNPs) para el uso como un sistema de liberación controlada de fármacos. Las nanopartículas se sintetizaron incorporando el éter metílico de poli(etilenglidol) al quitosano, y se prepararon a través de la gelación iónica entre el mPEG-quitosano y el ácido hialurónico (HA). Las nanopartículas así preparadas se probaron en su efectividad para la absorción y liberación de 7-etil-10-hidroxicamtotecina (SN-38) en forma de dispersiones líquidas. El absorbente hecho a partir de las nanopartículas se caracterizó mediante espectroscopía infrarroja de transformada de Fourier (FT-IR), calorimetría diferencial de barrido (DSC) y microscopía electrónica de barrido (SEM). Se encontró que la isoterma de adsorción d