Oral rabies immunization of swine: use of Vnukovo-32/107 vaccination strain

The apathogenicity and antigenic activity of the live oral rabies vaccine prepared from the strain Vnukovo-32/107 were evaluated in two experiments carried out on non-target species - swine (domestic swine Sus scrofa domestica and wild boar Sus scrofa). The Vnukovo-32/107 vaccination strain has been used to produce oral rabies vaccine Kamark for immunization of free living carnivores. Wild boars compete with foxes for acceptance of baits distributed in the field. The tested vaccination strain for oral application (including very high doses) proved to be apathogenic to non-target species - swine. Detection and quantification of rabies antibodies were carried out by rapid fluorescence focus inhibition test (RFFIT), ELISA and virus neutralization test (VNT) on mice on days 30 and 90 post-immunization. The value of 0,5 IU/cubic cm was used as a positivity criterion. Antirabic antibodies were detected in more than 50 % of animals on day 30 post-immunization. The results obtained show good antigenic activity of the live oral rabies vaccine prepared from the strain Vnukovo-32/107.