FRACTIONATION OF URINARY GLYCOPEPTIDE BY DEAE SEPHADEX A-50 COLUMN CHROMATOGRAPHY

Glycopeptides in normal human urine were assayed by determining the carbohydrate content of material which remained after proteolysis. Fractionation of nondialyzable glycopeptide by DEAE Sephadex A-50 column chromatography yielded five fraction which were characterized by an increase in peptide and sialic acid as the molarity of Tris-HCl buffer increased. The sugar components consisted of galactose, mannose, fucose, sialic acid, glucosamine and galactosamine.