New Methodology for Non-destructive Identification of Body Fluid for Forensic Purposes
The purpose of this research study was to test and subsequently validate a method based on the identification of biological fluids by nondestructive analysis, attributing phenotypic characteristics to biological stains. Specifically, a new methodology for non-destructive forensic histological analys...
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Veröffentlicht in: | Arab journal of forensic sciences & forensic medicine 2024-07, Vol.6 (Special Issue), p.117-124 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The purpose of this research study was to test and
subsequently validate a method based on the identification
of biological fluids by nondestructive analysis, attributing
phenotypic characteristics to biological stains. Specifically,
a new methodology for non-destructive forensic histological
analysis using Alternative Light Source 450 nm + orange
filters was developed and validated - also testing its analytical
efficiency and inherent degree of probabilistic error.
The validation process has been carried out through the
creation of a standard database of known samples, in which
were included blood-stains, semen stains, saliva stains
and urine stains. Histological profiling with the observation
of given parameters was included in the study such as
fluorescence/absorbance, stain’s shape, intensity of the
signal, concentration of the signal etc.
160 reference samples were produced, which were used
as a comparison method for subsequent histological profiling
activities. Through a method validation procedure developed
on four different tests of the technically investigated samples,
specific useful ranges were identified for histological-forensic
diagnosis of the biological stains, both known in the reference
sample and unknown samples. Careful observation of the
biological traces revealed several phenotypic characteristics,
through which the study took shape. In conclusion, this
experimental work made it possible to design and validate a
a non-destructive analytical methodology, which is particularly
useful in the forensic field. Through this study is it possible to
identify the histology of stains founded on the findings with a
non-destructive analysis; furthermore it has to been specified
that, according to the data obtained, it is possible to identify
correctly the histological composition of the blood-stains but
the histological identification of stains from semen, saliva and
urine is still probabilistics. |
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ISSN: | 1658-6786 1658-6794 |
DOI: | 10.26735/AXEM4254 |