CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY STUDIES OF CALCITE-ZINCATE NANOPARTICLES BY GREEN SYNTHESIS

Nanoparticles (Nps) are extremely useful in a extensive choice of industries, together with electronics, the environs, cosmetics, material science, and medicinal systems, among others. Calcium Carbonate (CaCO3) and Zinc Oxide (ZnO) nanomaterials have long been popular research topics. It is frequently used in dentistry applications due to its biocompatibility. The purpose of this study was to produce and describe calcium carbonate/zinc oxide nanoparticles (CaCO3/ZnO-NPs) prepared via precipitation method. The precipitation method has many advantages, including being more controllable and reproducible, as well as allowing for easy particle size control. Temperature and the calcination process both have an impact on the formation of nanoparticles. The calcination at high temperatures resulted in spherical-shaped particles, a reduction in aggregate size, and an increase in the crystalline nature of the NPs. Nitrates of calcium and zinc, sodium hydroxide were used, along with silk washed waste water containing sericin as a capping agent. CaCO3/ZnO-NPs were produced at 60°C using calcination (500°C). The antibacterial activities of the nanoparticles against gram-positive bacteria Staphylococcus aureus and Enterococcus faecalis, as well as gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, also, Fungus - Candida albicans were investigated, via the disk diffusion method. CaCO3/ZnO-NPs were successfully produced and exhibited a crystalline nanostructure, confirmed the elements of CaCO3/ZnO-NPs, and revealed that the CaCO3/ZnO-NPs obtained had an irregular spherical shape. The nanoparticles were found to be void of contaminants and organic. The absorption group at nm points out the occurrence of CaCO3, and the absorption group at 380 nm confirm the existence of ZnO. In addition, these CaCO3/ZnO-NPs have improved antimicrobial activity with potential applications in dentistry.