Mucoprotective and Mast Cell Stabilization Activities of Chromium (D-Phenylalanine)3 in Indomethacin-Induced Chronic Enterocolitis in Rats: In vitro Anti-Cancer Activity Using Caco-2 Cell Line

Objective The current research work was intended to establish the positive effects of chromium complex CrD-phe3 in indomethacin induced chronic enterocolitis. Additionally Caco-2 was used to elucidate the anti-cancer property of the title compound.Methodology Oral administration of indomethacin 1.5 mgkg twice quotidian for fourteen days was done to induce chronic enterocolitis in rats. Pre-treatment with chromium complex 30 amp 60 mugkg p.o for three days before induction was done and this continued up to the end of the study. Macroscopic scoring of the stomach jejunum and ileum was done to analyze the extent of damage and inflammation. Mesenteric mast cell degranulation counts serum and ileum tissue biochemical estimations and histopathological analysis were carried out. MTT assay was performed to identify the cytotoxicity of Cr D-Phe3 using Caco-2 cells.Results Oral administration of CrD-phe3 displayed a substantial reduction in the elevated macroscopical scores oxidative-nitrative stress biochemical parameters and mast cell degranulation induced by chronic treatment of indomethacin. The protective effect of the chromium complex was also established through histological inspection of the ileum. Further treatment of the title complex resulted in Caco-2 cell viability reduction and exhibited IC50 722.40 mM.Conclusion The pragmatic usefulness of the chromium complex might be owed to its antioxidative anti-inflammation anti-allergic and mucoprotective properties. In addition the title complex exhibited anticolorectal cancer.nbsp