In Vitro Propagation of Brassica Campestris Plants and a Trial of Refinement of Propagation by Cost Simulation

Micropropagation of Brassica campestris in vitro was studied by the laboratory procedure. Namely, multiple shoots were induced by culturing excised shoots from seedlings in modified Murashige and Skoog medium containing 50 μM BAP, 3% sucrose, and 0.8% agar. Multiple shoots were also successively induced from excised lateral shoots cultured in medium containing BAP 50 μM and IBA 1 μM at20 day intervals in vitro. Shoots multiplied about three times every 20 days. When excised single shoots 2-4 cm in length were cultured in Modified Murashige and Skoog's medium or Otsuka hydroponic culture nutrient containing 3% sucrose and 0. 8% agar, at least 90% shoots rooted in vitro. Planting-out of regenerated plants were successfully achieved via acclimation in vermiculite beds containing Otsuka hydroponic culture nutrient for 8 days. At least ninety % of regenerants were successfully planted out. Production cost of in vitro plants at 10, 000 level production was calculated to be 101. 3 yen each. Refinement of production procedure by simulation of production cost was discussed.