Apigenin-7-O-Β-D-Glucuronide Methyl Ester Isolated from Manilkara zapota Leaves

In nature there are numerous plants available with medicinal properties. Around 70 % of medicinal plants are found in tropical areas of India. The search for natural products and compounds derived from natural sources has played a vital role in drug discovery due to their pharmacological importance. Compounds isolated from botanical sources remain an important source of several clinically useful anti-inflammatory agents. Manilkara zapota is a large, evergreen forest tree belongs to family sapotaceae. It is commonly known as chiku (Hindi), sofeda (Bengali) sapodilla and sapoti (French), chickle tree, hase berry, tree potato (English). Manilkara zapota is a species of lowland rain forest. Manilkara zapota and its different parts have been traditionally used for alleviating inflammation related diseases such as arthritis, cancer and skin infections. The present study aims to isolate, structurally characterize and analyze the bioactive compound from Manilkara zapota by using chromatographic and spectrophotometric techniques on the basis of inhibitory effects on sPLA2 by activity guided fractionation of ethyl acetate extract of Manilkara zapota leaves. Among the six fractions (F1-F6) tested fraction-5 showed significant inhibitory effects on sPLA2 activity hence fraction -5 was further subjected to structural analysis for identification of bioactive compounds by using analytical techniques such as TLC, HPLC, FT-IR, LC-MS and 1H ,13C NMR studies. The isolated compound identified as apigenin-7-O-β-D-glucuronide methyl ester.