Exploring Antimicrobial Phytocompounds in Tanacetum dolicophyllum: A GC-MS Profiling Approach

Tanacetum dolicophyllum, known locally as tansies, exhibitng a plethora of challenging and noteworthy pharmacological properties. Aerial parts of T. dolicophyllum were utilized for extract preparation, and then their in-vitro antimicrobial activity was assessed against various organisms employing both the well diffusion method and MIC assay. The ethyl acetate extract of T. dolicophyllum was analyzed using gas chromatography-mass spectrometry (GC-MS) investigation to determine phytocomponents and investigate their antimicrobial activity. The result demonstrated that ethyl acetate extract exhibited good antimicrobial activity. The extract showed the highest efficacy against Klebsiella pneumoniae, Streptococcus agalactiae, S. pyogenes, Escherichia coli, and Candida albicans. Analysis revealed the presence of 37 components in the T. dolicophyllum extract, as determined by GC-MS investigation. The most abundant compounds include pentacosane, constituting 17.31 of the peak area% with a retention time (RT) of 24.603, and molecular formula C25H52; 2(3H)-Furanone, dihydro-3-[(7-methoxy1,3-benzodioxol-5-yl)methyl]-4-[(3,4,5-trimethoxyphenyl)methyl]- accounting for 6.43 peak area% with a RT of 31.829 and, molecular formula C23H26O8; 4-(1,3-benzodioxol-5-ylmethyl)dihydro-3-[(3,4,5-trimethoxyphenyl)methyl]- representing 5.76 peak area%, RT 28.409 and, molecular formula C22H24O7; Yangambin present at 5.44% of peak area, with an RT of 29.513 and, molecular formula C24H30O8; Chrysantenyl 2-methuylbutanoate constituting 4.60 of peak area%, RT 18.825, and molecular formula C15H24O2; Phytyl tetradecanoate with 3.22 area%, RT 31.284, and formula C34H66O2; and Tricosane accounting for 1.44 peak area%, with a RT of 18.228, and molecular formula C23H48. These compounds exhibit various medical potentials, suggesting the ethyl acetate extract of T. dolicophyllum may have diverse pharmacological applications.