Psidium guajava Linn Leaves Extracts: A Potential Digestive Enzyme Inhibitor in the Treatment of Obesity

Obesity is a major worldwide problem and is associated with metabolic disorders, including hyperglycemia and hyperlipidemia. Many researchers focus on herbal plants rich with polyphenols are potent inhibitors of digestive enzymes and could be used as antihyperlipidemic and antihyperglycemic and hence useful in obesity treatment. The present work deals with the determination of the inhibitory effect of Psidium guajava Linn (Guava) leaves extract on digestive enzymes. Our findings confirmed that guava leaf extracts inhibit two primary enzymes, α-amylase and α-glucosidase. Similarly, investigated pancreatic lipase inhibition potential. The leaf extracts of P. guajava demonstrated significant anti-obesity properties by inhibiting key enzymes associated with obesity development. The aqueous extract exhibited pancreatic lipase inhibition ranging from 19.25 to 38.51%, while the methanolic extract showed inhibition between 22.96 to 46.66%. Additionally, the aqueous extract displayed α-amylase inhibition ranging from 43.10 to 62.06%, and the methanolic extract showed inhibition between 48.27 to 62.06%. Moreover, the aqueous extract demonstrated α-glucosidase inhibition between 13.11 to 49.18%, and the methanolic extract displayed inhibition ranging from 29.50 to 60.65% for 200 to 1000 μg/mL concentrations. The inhibitory activity observed was dosedependent for all three enzymes.