Extraction and purification of klebocin from urine isolate Klebsiella pneumonia(k42)

From September 2016 to January 2017 ninety isolates of Klebsiella spp. have been collected from clinical sample(urine,sputum, wound swab, burn swab,and ear swab). There are three Klebsiella spp. were identified: 53 isolates of Klebsiella pneumoniae pneumoniae, 29 isolates of Klebsiella pneumoniae ozaenae, and 8 isolates of Klebsiella oxytoca. Cup assay method was used to determine capability of klebsiella spp. to produce bacteriocin. The results showed (7/36) 19.4% of isolates produced bacteriocin. Isolates number 42 of Klebsiella pneumoniae pneumoniae, which selected to extract and purify klebocin. Molecular weight of klebocin was 95 kd determined through gel filtration by using cepharose 6B gel. In this study antimicrobial activity was tested against different pathogenic bacteria, such as E. coli, Staphylococcus aureus, and Pseudomonas aeruginosa in different inhibition zones range from 11mm to 17mm.