Sequence-Tagged Site Markers in Grapevine and Citrus(Utilization of DNA-Marker for Breeding and Classification in Horticultural Plants, For Further Development of Horticulture in East Asia)

DNA markers based on PCR are becoming increasingly important in a wide range of applications including cultivar identification, phylogenetics and the construction of linkage maps for marker assisted breeding and map-based cloning of genes. We have investigated the utility of the microsatellite sequence-tagged site marker type because of the high level of associated polymorphism and simple genetics. For grapevine cultivar identification the marker type has been very successful. For phylogenetic analysis, studies with Citrus and related genera suggests that microsatellite length variation may be better suited to construct relatedness over small genetic distances rather than large genetic distances. The integration of trinucleotide microsatellite markers into a linkage map of Citrus has demonstrated the utility of this marker type for genetic analysis within wide intergeneric crosses and the potential to act as "anchor loci" to align linkage maps from different crosses and laboratories.