ENTRAPMENT OF SEED EXTRACT OF NIGELLA SATIVA INTO THERMOSENSITIVE (NIPAAM–CO–VP) CO-POLYMERIC MICELLES AND ITS ANTIBACTERIAL ACTIVITY

Thermosensitive N-Isopropyl acryl amide-N-Vinyl 2-pyrollidone (NIPAAm-VP) co-polymeric micelle synthesized by radical copolymerization and the extract of Nigella sativa is entrapped in this polymeric system to check the release of the bioactive compound and evaluated its antibacterial activity. The size of nanoparticles was measured 75-110 nm and 1 % drug is entrapped. In this work, the seed extract has been loaded into the polymeric micelle and its effectiveness has been evaluated against gram positive strain of Staphylococcus aureus. Bacillus subtilis and a gram negative strain of Escherichia coli. Nigella sativa loaded polymeric micelles found hundred times efficient in comparison of the naked one. Drug release from the polymeric micellar system at 37-42oC in different time intervals i.e. 30 min - 4 weeks is measured 7.14-66.66%. The above findings revealed that this thermosensitive polymeric system would more effectively release the drug into the body when infectious states are functional i.e. higher temperature and may have possibility to use as a novel drug delivery system for more herbal drugs for patient compliance.