Circular RNA Circ_0001017 Acts as ceRNA Adsorbing miR-197-3p to Regulate PNLIP Signaling and Affect the Proliferation, Apoptosis and Glycolysis of Pancreatic Ductal Adenocarcinoma Cells

To investigate the effects of circ_0001017 as a molecular sponge adsorbing miR-197-3p to regulate PNLIP signaling on the proliferation, apoptosis and glycolysis of pancreatic ductal adenocarcinoma (PDAC) cells, the expressions of circ_0001017, miR-197-3p and PNLIP in PDAC tissues and cells were detected. The dual-luciferase reporter assay and RNA-binding protein immunoprecipitation assay were used to verify the targeting relationship between miR-197-3p and circ_0001017 or PNLIP. The expressions of circ_0001017/miR-197-3p/PNLIP in cells were interfered and they were grouped. The proliferation activity and apoptosis of the cells were measured by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry, respectively. Glucose, lactate, and ATP detection kits were used to assess glucose consumption, lactate production, and ATP levels. Compared with paracancerous tissues, miR-197-3p expression in PDAC tissues was significantly up-regulated, while circ_0001017 and PNLIP expressions were decreased (all P