Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract

Probiotics are living microorganisms able to reach the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be obtained from lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. pH resistance testing of LAB was performed by introducing diluted bacteria into buffer solutions with different pH and a predetermined incubation time. The design used a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.