Occurrence of Enterocin Genes in Enterococci from Slovak Milk Product Žinčica

Žinčica is a popular Slovak dairy product made from ewes’ milk. It is a by-product resulting during ewes’ lump cheese processing. Microbiota in Žinčica have rarely been studied, especially enterococci; however, they can produce beneficial substances – bacteriocins. In this study, the presence of ( ) genes were analysed in enterococci from Žinčica and partially also the inhibition activity. Samples of Žinčica were collected from different agrofarms producing ewes’ lump cheese (34) in Central Slovakia. In the enterococci tested, P gene was the most frequently detected (in 6 out of 7 enterococci), followed by A and L50B genes. B gene was detected only in 30E1. On the other hand, 31E2 did not contain genes, although it showed inhibition activity against the indicator strains EA5, SA5, CCM4699 (inhibition zone sizing up to 20 mm). 30E1 contained genes of four ; however, it showed no inhibition activity. Growth of the four indicators was inhibited due to the antimicrobial activity of 32E1 with P gene detection. This is the first study reporting on the occurrence of genes in enterococci from Žinčica.