Isolation and Identification of Lactic Acid Bacteria in Wine Production by MALDI-TOF MS Biotyper

The aim of this study was to identify lactic acid bacteria (LAB) in grapes, must and wines. A total amount of 90 samples including grape ( = 30), must (no = 30) and wine (no = 30) were collected from vineyards in Slovakia. LAB were used cultured on MRS agar with subsequent confirmation with MALDI-TOF mass spectrometry (Bruker Daltonics). Altogether, 904 isolates were identified. Members of the family Lactobacillaeceae were the most abundant in grape (60%), must (46%) and wine (51%). , , , and genera and 27 species of LAB were isolated from the examined samples. spp. was the most abundant species in grape, must and wine.