Effects of Lipopolysaccharide from Periodontopathic Bacteria and Interleukin-1 on Collagen Synthesis in Cultured Human Fibroblasts

We studied the effects of lipopolysaccharide LPS) from Porphyromonas (Bacteroides) gingivalis and Prevotella (Bacteroides) intermedia, and interleukin-1 (IL-1) on production of collagen in cultured human fibroblasts. Fibroblasts were obtained from healthy gingiva, inflamed gingiva and healthy periodontal ligament. After the cells had reached confluence, they were incubated with LPS (final concentration: 10 μg/ml) or IL-1 (final concentration: 0.5, 5 μg/ml) or LPS (10 μg/ml) +IL-1 (0.5, 5 μg/ml) for up to 48 hours. Collagen levels in culture media were measured using Procollagen type IC-Peptid EIA Kit. Collagen levels were increased by stimulation with LPS or IL-1. Collagen levels were greatly increased by stimulation with LPS IL-1 as compared to those of stimulated by LPS or IL-1 alone. From these findings, it is suggested that LPS and/or IL-1 may promote collagen synthesis of fibroblasts in periodontal tissue in vivo.