Characteristics of Human Alveolar Bone-Derived Cells III. Alteration of Phosphatase Activities during Bone Nodule Formation

The purpose of this study was to elucidate the mineralizing process of nodules formed by human alveolar bone-derived cells, and changes in phosphatase activities following culture periods. The cells were obtained from human alveolar bone fragments, after the fragments had been treated with bacterial...

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Veröffentlicht in:Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 1992/09/28, Vol.34(3), pp.665-672
Hauptverfasser: SUZUKI, Kuniharu, AKIYAMA, Hirotaka, KATO, Takashi, KOSUGE, Kazuhiro, FUJIKAWA, Kenji, MURAI, Seidai, SUZUKI, Naoto, MAENO, Masao, OTSUKA, Kichibee, SUZUKI, Kantaro
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Sprache:jpn
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Zusammenfassung:The purpose of this study was to elucidate the mineralizing process of nodules formed by human alveolar bone-derived cells, and changes in phosphatase activities following culture periods. The cells were obtained from human alveolar bone fragments, after the fragments had been treated with bacterial collagenase as described in a previous paper. The mineralized nodule formation and two phosphatase activities of the cells were determined on culture days 10, 14, 28 and 42 after seeding; the enzymes were alkaline phosphatase (ALPase) and acid phosphatase (ACPaes). Cell growth showed logarithmic patterns from day-3 to 10, and reached to confluence on day-14. After confluence, cell numbers increased gradually until day-42. The nodules began to form on day-14, and a small number of nodules, slightly stained by alizalin red S, appeared on day-28, indicating the initiation of the mineralization process. To determine phosphatase activity per 104 cells, enzyme assay was directly carried out using the cells in each well of a 96-well microplate. ALPase activity increased daily, and the highest activity was shown on culture day-14, coinciding with nodule formation. On day-28 and 42, ALPase activity was drastically decreased. On the other hand, ACPase activity was about one tenth of ALPase activity during the culture periods and about one fifteenth on culture day-14.
ISSN:0385-0110
1880-408X
DOI:10.2329/perio.34.3_665