Intestinal Crypt Survival and Total and Per Crypt Levels of Proliferative Cellularity Following Irradiation: Role of Crypt Cellularity

Mice were given whole-body exposures of 500 R, or abdomen-only exposures of 900 R. One or 4 days later, when crypt cellularity was either relatively low or high, respectively, a graded series of exposures was given, and crypt survival and total and per crypt levels of proliferative cellularity measu...

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Veröffentlicht in:Radiat. Res. 50: No. 3, 583-91(Jun 1972) 583-91(Jun 1972), 1972-06, Vol.50 (3), p.583-591
Hauptverfasser: Hagemann, Ronald F., Sigdestad, Curtis P., Lesher, S.
Format: Artikel
Sprache:eng
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Zusammenfassung:Mice were given whole-body exposures of 500 R, or abdomen-only exposures of 900 R. One or 4 days later, when crypt cellularity was either relatively low or high, respectively, a graded series of exposures was given, and crypt survival and total and per crypt levels of proliferative cellularity measured 3 days thereafter. Crypt survival was found to be markedly affected by crypt cellularity existing at the time of irradiation. Advantage may be taken of the compensatory proliferative intestinal response following a given radiation exposure to ameliorate somewhat, the efficacy of a subsequent exposure for crypt killing. A precise analysis of the relationship between proliferative and crypt progenitor compartment sizes was thwarted however, by an apparent increase in D0 of cells surviving the conditioning exposure. The difference in D0's was marginally significant when determined 3 days after 500 R, and not significant 1 or 4 days after 900 R. At high radiation exposures, the proliferation rate of cells in surviving crypts was markedly reduced, in spite of a continued stimulus for accelerated proliferation. This suggests that a condition relating to growth, possibly analogous to small colony formation seen in vitro, exists in the heavily irradiated mammalian intestine.
ISSN:0033-7587
1938-5404
DOI:10.2307/3573555