Sulphuryl Fluoride Fumigation: Effect on DNA Extraction and Amplification from Herbarium Specimens

Sulphuryl Fluoride Fumigation: Effect on DNA Extraction and Amplification from Herbarium Specimens

Dried leaf samples were exposed to sulphuryl fluoride (Vikane) during herbarium fumigation. DNA was extracted from fumigated leaf samples and from unfumigated controls. Both fumigated and control samples yielded good quality DNA; DNA amplification yielded similar products from both sets of samples....

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Veröffentlicht in:Taxon 1999-08, Vol.48 (3), p.507-510
Hauptverfasser: Whitten, W. Mark, Williams, Norris H., Glover, Kenneth V.
Format: Artikel
Sprache:eng
Schlagworte:
Bromides
Fluorides
Fumigation
Herbaria
Methods and Techniques
Microwaves
Specimens
Taxa
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Beschreibung
Zusammenfassung:Dried leaf samples were exposed to sulphuryl fluoride (Vikane) during herbarium fumigation. DNA was extracted from fumigated leaf samples and from unfumigated controls. Both fumigated and control samples yielded good quality DNA; DNA amplification yielded similar products from both sets of samples. Vikane does not appear to degrade DNA present in herbarium specimens, and appears to be a non-destructive fumigant for pest control in herbaria.
ISSN:0040-0262
1996-8175
DOI:10.2307/1224563