Analysis of morphological traits of bird's foot trefoil plants cv. Bokor transformed with Agrobacterium rhizogenes

An efficient method for genetic transformation and shoot regeneration was achieved in bird's foot trefoil cv. Bokor using A. rhizogens. The transformed shoots were regenerated on hairy root segments in high frequency. After rooting and acclimation, transformed To plants were grown in experimental field. Analysis of morphological traits and chemical content in ten unintentionally chosen To bird's foot trefoil plants (genotypes no. 2 and no. 5) was performed. They were compared to those of control non-transformed plants. The traits as a number of stems per plant, length of internodes in longest stem, number of flowers per plant and plan high were very significant differed than the same traits in control plants, while there were no significant differences in the leaf area. No signs of the rol genes genotype and "T" phenotype were present. The transformed plants had significantly higher content of cellulose, while the protein and nitrogen contents of are in the range of control plants. Uspostavljen je efikasan metod za transformaciju zutog zvezdana cv. Bokor pomocu A. rhizogenes. Transformisani pupoljci su regenerisani na segmentima adventivnih korenova u visokoj frekvenci. Posle oziljavanja i aklimatizacije transformisane To biljke su gajene na eksperimentalnom polju. Izvrsena je analiza morfoloskih karakteristika deset slucajno odabranih To biljaka zutog zvezdana (genotipovi br. 2 i br. 5). One su uporedjivane sa istim osobinama kontrolnih, netransformisanih biljaka. Osobine kao sto su broj stabala po biljci, duzina internodija najduzeg stabla i broj cvetova po biljci su bile signifikantno razlicite od istih osobina kontrolnih biljaka, dok nisu utvrdjene znacajne razlike u povrsini listova. Nije bilo znakova prisustva genotipa sa rol genima. Transformisane biljke su imale povecan sadrzaj celuloze, dok je sadrzaj proteina i azota bio na nivou kontrolnih biljaka.