Induction and micropropagation potential of sugar beet haploids

The aim of research was obtaining sugar beet haploids via gyno-genesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cyto-kinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each sub-cultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes. Cilj istrazivanja je bio dobijanje haploida secerne repe putem ginogeneze i njihova mikropropagacije. Haploidi su dobijeni kulturom semenog zametka iz Cetrnaest diploidnih, monogermnih i muski fertilnih genotipova secerne repe. Ispitivani genotipovi su na podlogama sa razlicitom koncentracijom stimulatora rasta ispoljili razlicit potencijal za ginogenezu. Za dalja ispitivanja je odabrano osam haploida koji su nakon razvica prvog para listova postavljeni na podlogu za mikropropagaciju. Prisustvo citokinina u hranljivoj podlozi je stimulisalo pojavu i razvice bocnih pupoljaka u pazuhu listova, dok je kod nekih genotipova takodje doslo do pojave adventivnih pupoljaka. Porast broja biljaka tokom mikropropagacije nije bio kontinuiran iako je broj novostvorenih biljaka rastao posle svake sub-kultivacije. Razlike u intenzitetu multiplikacije biljaka pocele su da se znacajno ispoljavaju nakon Cetvrte subkultivacije. Testiranjem znacajnosti razlika korelacionih koeflcijenata, odnosno intenziteta mikropropagacije ispitivanih genotipova tokom sest subkultivacija, utvrdjeno je da se one medjusobno statisticki znacajno razlikuju.