Canine Parvoviral Enteritis - The Role of MMP-9 and TIMP-1 in the Pathogenesis of Intestinal Inflammation

Background: Canine parvoviral enteritis is a highly contagious infection in the intestines caused great morbidity and mortality in untreated dogs younger than 6 months. Matrix metalloproteinases consist of zinc- and calcium-dependent extracellular matrix-degrading endopeptidases that are tightly controlled by endogenous metalloproteinase tissue inhibitors. Canine parvoviral enteritis is common in Turkey. The aim of this study was to examine the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in natural canine parvoviral enteritis infection of 25 dogs diagnosed with parvoviral enteritis by clinical tests and histopathology. Materials, Methods & Results: The study material consists of dog’s small intestine, which was brought to Cumhuriyet University Faculty of Veterinary Medicine pathology department for necropsy and diagnosed with parvoviral enteritis. This investigation was suported by the Comission of Scientific Research Projects of Cumhuriyet University (Project No: V-086). For the study, sections of 5 μm were taken from small intestine blocks consisting of duodenum, jejunum and ileum, fixed in 10% buffered formalin solution and embedded in paraffin, and stained with hematoxylin-eosin and MMP-9 and TIMP-1 antibodies using immunohistochemical procedure. On histopathology, shedding and blunting of the villi epithelium, severe mononuclear inflammation in the lamina propria and locally enlarged crypts with lymphocytolysis in peyer's patches were noted in the ileum. Immunohistochemically, strong expression for MMP-9 and moderate expression for TIMP-1 were observed in the crypt epithelium and inflammatory cells in the small intestines of infected animals compared controls (P < 0.001). Discussion: In the present study, immunohistochemical expressions of MMP-9 and TIMP-1 in intestinal tissues were investigated in canine parvoviral enteritis, which is an important viral disease in veterinary medicine. Statistically strong expression for MMP-9 and moderate expression for TIMP-1 were observed in the crypt epithelium and inflammatory cells in the small intestines of infected animals. As a result, high levels of MMP-9 may be one of the factors that trigger the inflammatory process in the disease. It is thought that the increase in MMP-9 may be directly proportional to the severity of inflammation in the tissue. In addition, it is suggested that the level of its inhibitor, TIMP-1, may increase at similar