Anti-Osteoclastic Effect of Zinc Studied in MCF-7 Induced Osteoclastogenesis

Bone which a key structural support of the body, undergoes dynamic micro structural remodelling all over life to control automatic stress and calcium requirement in the body. A number of risk factors including oxidative stress, apoptosis and abnormal intracellular Ca2+ metabolism have been postulated to play a function in the inception and progress of bone osteolysis. Cancer cells establish a tight relationship with the host tissue, secreting factors that stimulate or inhibit bone cells, receiving signals generated from the bone remodelling activity, and displaying some features of bone cells. This interplay between tumour and bone cells alters the physiological bone remodelling, leading to the generation of a vicious cycle that promotes bone metastasis growth. Zinc is one of the most relevant minerals to human health, because of its antioxidant properties. The present study was aimed to investigate protective role of zinc against bone metastasis. In the present study, TRAP positive multinucleated cell count was low compared to CM treated cells. Zinc treatment suppressed MCF-7 induced mRNA levels of cytoplasmic 1 (Nfatc1), TRAP and Cathepsin-K. Hence, it can be concluded that zinc decreases osteoclastogenesis induced by MCF-7 cells. Keywords: Oxidative stress; Metastasis; Bone remodelling; Zinc.