ANTIOXIDANT ACTIVITY (PHENOL AND FLAVONOID CONTENT ) OF THREE DIFFERENT CULTIVARS OF PIPER BETLE L. (PIPERACEAE)

In the present study, an attempt has been made for the estimation of total phenol and flavonoid content and their radical scavenging properties using Piper betle (L.) leaves. In that, Cultivars i.e., Nov Bangla (NB), Sirugamani-1 (SGM-1) and Halisar Sanchi (HS) were selected for this study. The total phenolic content was ranged from 95.04 to 127.33 mg/100g equivalent to gallic acid and flavonoids were ranged from 51.72 to 61.08 mg/ 100g equivalent to standards of Catechin. In vitro antioxidant activity was estimated using 1,1-diphenyl-2-picryl hydrazyl (DPPH), free radical scavenging activity, improved ABTS radical cation decolorization assay and ferric reducing antioxidant power (FRAP) assay. Among all the cultivars, The highest Phenol content (93.79%) was observed for Sirugamani-1 by DPPH method and highest Phenol content (96.12% & 6791.86 (µg/g) was obtained for Halisar Sanchi by ABTS assay and FRAP activity respectively. The study revealed that the leaves of Piper betle (L.) has higher amount of antioxidant activity and it could be used for any novel drug preparation.