In vitro plant regeneration system for common bean ( Phaseolus vulgaris ): effect of N6-benzylaminopurine and adenine sulphate
A method for regeneration of the commercially important common bean ( Phaseolus vulgaris ) using N6-benzylaminopurine(BAP) and adenine sulphate (AS) was established. Embryogenic axes of the Costa Rican common bean cultivars Bribrí, Brunca, Guaymí, Huetar and Telire were cultured on Murashige and Sko...
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Veröffentlicht in: | Electronic Journal of Biotechnology 2010-03, Vol.13 (1) |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A method for regeneration of the commercially important common bean (
Phaseolus vulgaris ) using N6-benzylaminopurine(BAP) and adenine
sulphate (AS) was established. Embryogenic axes of the Costa Rican
common bean cultivars Bribrí, Brunca, Guaymí, Huetar and
Telire were cultured on Murashige and Skoog medium supplemented with
100 mgl-1 myo-inositol, 1 mgl-1 thiamine, 30 gl-1 sucrose, BAP (0, 5
and 10 mgl-1), AS (0, 20 and 40 mgl-1) and 8 gl-1 agar. Regardless of
the concentration of BAP and AS in the induction medium, the number of
shoots and leaves differed significantly among the common bean
cultivars evaluated. The higher average of shoots was obtained for
Brunca > Telire > Bribrí > Guaymí > Huetar.
Moreover, independently of the cultivar, the induction medium
supplemented with 5 mgl-1 BAP and 20 or 40 mgl-1 AS resulted in the
higher average of shoots formation. Culture of Bribrí, Brunca,
Guaymí, Huetar and Telire embryogenic axes on induction medium
supplemented with different BAP and AS resulted in a differential
response. Successful acclimatization of common bean in vitro plants
were achieved in the greenhouse, and plants appeared morphologically
normal. The regeneration system developed in this investigation for
this important crop could be a useful tool for the genetic modification
through mutagenesis or genetic transformation. |
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ISSN: | 0717-3458 0717-3458 |
DOI: | 10.2225/vol13-issue1-fulltext-7 |