BACTERIAL DNA EXTRACTION METHOD FOR THE DETECTION OF HELICOBACTER PYLORI FROM HUMAN FECAL SAMPLES
Objective: The work proposed the implementation of a method of DNA extraction for the detection of the pathogen from 50 stool samples. Methods: A method of DNA extraction with Chelex resin was applied and then detected by conventional polymerase chain reaction (PCR). Results: By PCR, 11 samples were...
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| Veröffentlicht in: | Asian journal of pharmaceutical and clinical research 2020-04, p.38-40 |
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| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | Objective: The work proposed the implementation of a method of DNA extraction for the detection of the pathogen from 50 stool samples.
Methods: A method of DNA extraction with Chelex resin was applied and then detected by conventional polymerase chain reaction (PCR).
Results: By PCR, 11 samples were positive for Helicobacter pylori.
Conclusions: The Chelex extraction methodology allows obtaining DNA with quality necessary to be detected by PCR, making it a fast methodology for its diagnostic application. |
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| ISSN: | 0974-2441 0974-2441 |
| DOI: | 10.22159/ajpcr.2020.v13i6.37344 |