CYTOPROTECTIVE ACTIVITY OF ETHANOL FRACTION OF COLEUS AMBOINICUS LOUR. LEAVES AGAINST VERO CELLS INDUCED BY H2O2

Objective: The objective of this study is to assess the cytoprotective activity of Coleus amboinicus Lour. leaves ethanol fraction (EtF) in Vero cells which induced by hydrogen peroxide (H2O2).Methods: Cytoprotective activity was determined using 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis activity was analyzed by flow cytometry, and expression of radical spesies oxygen (ROS) was observed by imunocytochemistry.Result: The EtF (100 μg/mL) was showed the highest percent viability 74.42±0.28% using MTT method. Furthermore, the EtF was increased viability of Vero cell after induced with 0.8 mM H2O2 and EtF was decreased the expression of ROS.Conclusion: Based on the description, EtF has cytoprotective activity towards Vero cells which induced by 0.8 mM H2O2 .