DEVELOPMENT OF ANALYTICAL METHOD FOR ESTROGENS IN WASTEWATER USING LC/MS/MS

Estrogens are deemed to be important substances that may give estrogenic effects on fish in the water environment due to their occurrence in river water and wastewater. Therefore, we developed a novel analysis method for natural estrogens (E2, E1) and synthetic estrogen (EE2) in wastewater by LC/MS/MS. Detection limit and recovery ratio of the estrogens in this method ranged 0.12-0.24ng/l and 90-105% respectively. We measured estrogens concentration in the sewage treatment process by using this method. The results suggest that E2 is dehydrated in sewage treatment process, and is changed into E1. Such a dehydration was remarkablely observed in the aeration tank.