THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY
In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA. In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of...
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| Veröffentlicht in: | Japanese Journal of Clinical Immunology 1983/06/30, Vol.6(3), pp.145-152 |
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| container_title | Japanese Journal of Clinical Immunology |
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| creator | Kazuta, Yasuhito Ikeda, Keizo Hashimoto, Kiyoyasu Horiuchi, Atsushi |
| description | In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA. In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of DNA in the immune complexes in sera of SLE with active disease (4.43±2.10μg/ml) was significantly greater than that of SLE with inactive disease (1.20±1.15μg/ml, P |
| doi_str_mv | 10.2177/jsci.6.145 |
| format | Article |
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In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of DNA in the immune complexes in sera of SLE with active disease (4.43±2.10μg/ml) was significantly greater than that of SLE with inactive disease (1.20±1.15μg/ml, P<0.01). In addition, that of SLE correlated with the titer of anti-DNA antibodies and serum complement activity, but had no relationship with proteinuria. On the clinical coarse, that decreased significantly after prednisolone therapy. On these results, it was suggested that the fluorophotometry was useful to detect DNA-anti-DNA complexes in sera from SLE patients.</description><identifier>ISSN: 0911-4300</identifier><identifier>EISSN: 1349-7413</identifier><identifier>DOI: 10.2177/jsci.6.145</identifier><language>eng</language><publisher>The Japan Society for Clinical Immunology</publisher><subject>anti-DNA antibody ; DABA ; DNA-anti-DNA complex ; Fluorophotometry ; SLE</subject><ispartof>Japanese Journal of Clinical Immunology, 1983/06/30, Vol.6(3), pp.145-152</ispartof><rights>The Japan Society for Clinical Immunology</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids></links><search><creatorcontrib>Kazuta, Yasuhito</creatorcontrib><creatorcontrib>Ikeda, Keizo</creatorcontrib><creatorcontrib>Hashimoto, Kiyoyasu</creatorcontrib><creatorcontrib>Horiuchi, Atsushi</creatorcontrib><title>THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY</title><title>Japanese Journal of Clinical Immunology</title><addtitle>Jpn. J. Clin. Immunol.</addtitle><description>In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA. In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of DNA in the immune complexes in sera of SLE with active disease (4.43±2.10μg/ml) was significantly greater than that of SLE with inactive disease (1.20±1.15μg/ml, P<0.01). In addition, that of SLE correlated with the titer of anti-DNA antibodies and serum complement activity, but had no relationship with proteinuria. On the clinical coarse, that decreased significantly after prednisolone therapy. On these results, it was suggested that the fluorophotometry was useful to detect DNA-anti-DNA complexes in sera from SLE patients.</description><subject>anti-DNA antibody</subject><subject>DABA</subject><subject>DNA-anti-DNA complex</subject><subject>Fluorophotometry</subject><subject>SLE</subject><issn>0911-4300</issn><issn>1349-7413</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNo9j01PAjEQhhujiYhe_AV7Nin2c0sPHhYsQrJLCSyJnJrdfigEP7Ll4r-3oOEwmXkzz0zyAHCP0YBgIR530W4H-QAzfgF6mDIJBcP0EvSQxBgyitA1uIlxhxDlVLAeeKqnKqtUsVovVaXmdaYn2fO8gMW8nsE0ZGNdLUr1qlbZaJNNyrVe6sVU17pS9XJzC65Cs4_-7r_3wXqi6vEUlvplNi5KaAkXHBLuLPHM5nmQ3MlcMuacaz1xwzYBwabQYulywUiwvg3BDnkrCBPMC2QR7YOHv7-2-4qx88F8d9uPpvsxGJmjuDmKm9wk8QSP_uBdPDRv_ow23WFr9_6EYimGCaenSkfnpX1vOuM_6S-qOV7c</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Kazuta, Yasuhito</creator><creator>Ikeda, Keizo</creator><creator>Hashimoto, Kiyoyasu</creator><creator>Horiuchi, Atsushi</creator><general>The Japan Society for Clinical Immunology</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1983</creationdate><title>THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY</title><author>Kazuta, Yasuhito ; Ikeda, Keizo ; Hashimoto, Kiyoyasu ; Horiuchi, Atsushi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2575-25dc2e4c66f95d96944dddbe2d8b257fcddbb19d6742fcebffc85b72474e70c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>anti-DNA antibody</topic><topic>DABA</topic><topic>DNA-anti-DNA complex</topic><topic>Fluorophotometry</topic><topic>SLE</topic><toplevel>online_resources</toplevel><creatorcontrib>Kazuta, Yasuhito</creatorcontrib><creatorcontrib>Ikeda, Keizo</creatorcontrib><creatorcontrib>Hashimoto, Kiyoyasu</creatorcontrib><creatorcontrib>Horiuchi, Atsushi</creatorcontrib><collection>CrossRef</collection><jtitle>Japanese Journal of Clinical Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kazuta, Yasuhito</au><au>Ikeda, Keizo</au><au>Hashimoto, Kiyoyasu</au><au>Horiuchi, Atsushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY</atitle><jtitle>Japanese Journal of Clinical Immunology</jtitle><addtitle>Jpn. J. Clin. Immunol.</addtitle><date>1983</date><risdate>1983</risdate><volume>6</volume><issue>3</issue><spage>145</spage><epage>152</epage><pages>145-152</pages><issn>0911-4300</issn><eissn>1349-7413</eissn><abstract>In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA. In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of DNA in the immune complexes in sera of SLE with active disease (4.43±2.10μg/ml) was significantly greater than that of SLE with inactive disease (1.20±1.15μg/ml, P<0.01). In addition, that of SLE correlated with the titer of anti-DNA antibodies and serum complement activity, but had no relationship with proteinuria. On the clinical coarse, that decreased significantly after prednisolone therapy. On these results, it was suggested that the fluorophotometry was useful to detect DNA-anti-DNA complexes in sera from SLE patients.</abstract><pub>The Japan Society for Clinical Immunology</pub><doi>10.2177/jsci.6.145</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0911-4300 |
| ispartof | Japanese Journal of Clinical Immunology, 1983/06/30, Vol.6(3), pp.145-152 |
| issn | 0911-4300 1349-7413 |
| language | eng |
| recordid | cdi_crossref_primary_10_2177_jsci_6_145 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | anti-DNA antibody DABA DNA-anti-DNA complex Fluorophotometry SLE |
| title | THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY |
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