THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY

THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY

In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA. In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of...

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Veröffentlicht in:Japanese Journal of Clinical Immunology 1983/06/30, Vol.6(3), pp.145-152
Hauptverfasser: Kazuta, Yasuhito, Ikeda, Keizo, Hashimoto, Kiyoyasu, Horiuchi, Atsushi
Format: Artikel
Sprache:eng
Schlagworte:
anti-DNA antibody
DABA
DNA-anti-DNA complex
Fluorophotometry
SLE
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Zusammenfassung:In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA. In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of DNA in the immune complexes in sera of SLE with active disease (4.43±2.10μg/ml) was significantly greater than that of SLE with inactive disease (1.20±1.15μg/ml, P
ISSN:0911-4300
1349-7413
DOI:10.2177/jsci.6.145