Urinary proteome profile of renal papillary necrosis in experimental model rats

We aimed to identify novel biomarkers using toxicoproteomics to profile the urinary proteome of renal papillary necrosis (RPN) in experimental model rats. RPN was induced in rats by a single intraperitoneal injection of 2-bromoethylamine hydrobromide (BEA). Urinary proteins from four groups (control group; BEA-treated but no RPN; BEA-treated with RPN but no increase in blood urea nitrogen [BUN]; and BEA-treated with RPN and increase in BUN) were pooled. Relative quantitation of pooled urinary proteins was then performed by two-dimensional liquid chromatography-tandem mass spectrometry with the isobaric labeling technique. We found 77 proteins were changed in RPN model rat urine.