Induction of Polyphenol Oxidase in Walnut and Its Relationship to the Pathogenic Response to Bacterial Blight
Plants respond to pathogens with both active and passive defense mechanisms. These defense responses include the induction of defense or defense-related genes such as polyphenol oxidase (PPO) and pathogenesis-related (PR) proteins. The role of PPO in the interaction between bacterial blight [ Xantho...
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Veröffentlicht in: | Journal of the American Society for Horticultural Science 2016-03, Vol.141 (2), p.119-124 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Plants respond to pathogens with both active and passive defense mechanisms. These defense responses include the induction of defense or defense-related genes such as polyphenol oxidase (PPO) and pathogenesis-related (PR) proteins. The role of PPO in the interaction between bacterial blight [
Xanthomonas arboricola
pv.
juglandis
(
Xaj
)] and walnut (
Juglans regia
) was studied.
JrPPO-1
and
P14a
genes were identified in two walnut cultivars, Chandler and Serr, using standard polymerase chain reaction (PCR) to understand their inducible ability in response to
Xaj
. ‘Serr’ and ‘Chandler’ were inoculated with
Xaj
strain 417. PPO activity in leaves was assayed at 0, 24, 72, 96, 120, and 144 hours after inoculation. Results showed a steady increase in activity commencing within 24 hours of inoculation. Increase in PPO activity was close to 2-fold greater in ‘Chandler’ than in ‘Serr’ at all time points examined. Real-time PCR analysis showed differences between cultivars in PPO gene expression. The
JrPPO-1
gene was highly expressed in both cultivars 24 hours after inoculation but expression in ‘Serr’ was much greater than in ‘Chandler’. Significant expression of
P14a
gene was observed in both cultivars within 24 hours. Expression in ‘Serr’ was strong and maximized with a significant increase at 96 hours. Expression in ‘Chandler’ was far weaker than ‘Serr’ at 24 hours and did not increase further. Our results imply that the walnut–bacterial blight interaction induces the expression of
JrPPO-1
and
P14a
as well as the activity of PPO. |
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ISSN: | 0003-1062 2327-9788 |
DOI: | 10.21273/JASHS.141.2.119 |