Hot Water and Elevated CO2 Effects on Proline and Other Compositional Changes in Relation to Postharvest Chilling Injury of `Marsh' Grapefruit

Heat treatments and exposure to elevated CO 2 are known to reduce the incidence of chilling injury on grapefruit. In the current study, `Marsh' grapefruit ( Citrus paradisi Macf.) were harvested on 17 Jan. or 22 Mar. 1996 and exposed to hot water (HW) dips (48 °C for 120 minutes) or exposed to controlled atmosphere (CA) of 10% or 16% CO 2 during the first 3 weeks of an 8-week cold storage period (4.5 °C) to test their effects on the development of peel pitting (i.e., chilling injury) and proline and other compositional changes of the peel and juice. All HW and CA treatments from both harvests greatly reduced the development of peel pitting compared to the control. These treatments were also associated with lower average proline levels in the flavedo during storage. This suggests that HW and elevated CO 2 may reduce chilling-induced peel pitting by facilitating proline metabolism in grapefruit flavedo tissue. HW and CA treatments resulted in higher peel total soluble and nonreducing sugar levels, but effects on peel reducing sugar and free amino acid concentrations were not consistent. In the juice, HW reduced titratable acidity (TA) concentrations while CA tended to increase both TA and ascorbic acid concentrations. Compared to the control, CA resulted in a slight decrease in total soluble solids during storage, while the effect of HW was inconsistent.