Chromatography of p-hydroxyphenacyl esters of saturated fatty acids: Analytical studies on fatty acids. II

Paper-, thin layer-, and column-chromatography of p-hydroxyphenacyl esters of saturated fatty acids were investigated. In the paper chromatography, each p-hydroxyphenacyl esters of C2C20 fatty acids gave different Rf values depending on the number of carbon atoms of the acid. The solvent systems were 30 and 80 per cent aqueous acetone (0.1 N NaOH). The separation of the esters of C2C12 fatty acids was achieved by the silica-gel thin layer chromatography with acetic acid-cyclohexane as the solvent system. Using the same solvent, cellulose powder column chromatography was successful for separating the esters of C2C14 fatty acids. The esters could be detected with phenolic color reactions.