DETERMINATION OF ENKEPHALINS IN FETUS BRAIN TISSUES BY MEANS OF HPLC WITH PRE-COLUMN FLUORESCENCE DERIVATIZATION

DETERMINATION OF ENKEPHALINS IN FETUS BRAIN TISSUES BY MEANS OF HPLC WITH PRE-COLUMN FLUORESCENCE DERIVATIZATION

A HPLC method for the simultaneous determination of neuropeptides in complicated biological samples has been presented. 9-fluorenylmethyl chloroformate has been used as a pre-column fluorescent reagent. The linearity of this method is satisfactory in the range of 1-100 pmol. The concentrations of en...

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Veröffentlicht in:Analytical Sciences 1991, Vol.7(Supple), pp.951-954
Hauptverfasser: Zhao, Rui, Liu, Guo-quan, Feng, Fang-bo, Zhang, Ren-en
Format: Artikel
Sprache:eng
Schlagworte:
enkephalin
fluorescence detection
FMOC-CL
HPLC
human fetus brain
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Zusammenfassung:A HPLC method for the simultaneous determination of neuropeptides in complicated biological samples has been presented. 9-fluorenylmethyl chloroformate has been used as a pre-column fluorescent reagent. The linearity of this method is satisfactory in the range of 1-100 pmol. The concentrations of enkephalins in different regions of human fetus brain has been determined using HPLC method and fell in the range of several to hundreds pmol per gram wet tissue.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.7.Supple_951