STRONGLY BASIC ANION EXCHANGE RESINS PREPARED FROM DIFFERENT POLYMER MATRICES AND THEIR APPLICATION TO SEPARATION OF PROTEINS IN HIGH PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY

STRONGLY BASIC ANION EXCHANGE RESINS PREPARED FROM DIFFERENT POLYMER MATRICES AND THEIR APPLICATION TO SEPARATION OF PROTEINS IN HIGH PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY

Quaternized dimethylaminomethylstyrene-ethylene glycol dimethacrylate(EGDMA) (PDAMS-Q) and chloromethylstyrene-EGDMA(PCMS-Q) were prepared as column packings having the same functional groups for HPIEC of proteins. These resins prepared from different polymer matrices varied from each other in the r...

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Veröffentlicht in:Analytical Sciences 1991, Vol.7(Supple), pp.187-190
Hauptverfasser: ETOH, TAKASHI, MIYAZAKI, MIKA, IWATAKE, MIYUKI, HARADA, KUMIKO, NAKAYAMA, MORIO, SUGII, ATSUSHI
Format: Artikel
Sprache:eng
Schlagworte:
HPLC
ion-exchange chromatography
protein
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Zusammenfassung:Quaternized dimethylaminomethylstyrene-ethylene glycol dimethacrylate(EGDMA) (PDAMS-Q) and chloromethylstyrene-EGDMA(PCMS-Q) were prepared as column packings having the same functional groups for HPIEC of proteins. These resins prepared from different polymer matrices varied from each other in the retention behavior of proteins. PDAMS-Q column was suitable for analysis of acidic proteins, but PCMS-Q eluted all proteins at void volume.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.7.Supple_187