Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus

Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of game-togenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-leng...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Zoological Science 2001-05, Vol.18 (4), p.559-565
Hauptverfasser: Unuma, Tatsuya, Okamoto, Hiroyuki, Konishi, Kooichi, Ohta, Hiromi, Mori, Katsuyoshi
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 565
container_issue 4
container_start_page 559
container_title Zoological Science
container_volume 18
creator Unuma, Tatsuya
Okamoto, Hiroyuki
Konishi, Kooichi
Ohta, Hiromi
Mori, Katsuyoshi
description Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of game-togenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-length cDNA encoding vitellogenin (Vg; the MYP precursor in the coelomic fluid), and investigated its expression in various tissues of immature adults. The nucleotide sequence of Vg contains an open reading frame of 4050 bp encoding 1349 amino acids. The deduced amino acid sequence near the N-terminal showed 25% homology to the vertebrate transferrin family. Vitellogenin mRNA was detected in the ovary, testis, stomach, intestine and rectum by Northern blot analysis, with the highest level of mRNA expression in the gonad. Weak expression was also detected in the esophagus and coelomocytes by RT-PCR. In situ hybridization demonstrated that nutritive phagocytes, which exclusively fill the lumina of the immature gonad, contained Vg mRNA. These results suggested that the MYP stored in the immature gonads is synthesized and accumulated mainly within the nutritive phagocytes.
doi_str_mv 10.2108/zsj.18.559
format Article
fullrecord <record><control><sourceid>pier_cross</sourceid><recordid>TN_cdi_crossref_primary_10_2108_zsj_18_559</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>http_www_pieronline_jp_content_article_0289_0003_18040_559</sourcerecordid><originalsourceid>FETCH-LOGICAL-b3759-f8ecae7b57fa4e95611d7d528ef7f29fbcc6d3ff8d6cfafeb76c2f06be3c08bd3</originalsourceid><addsrcrecordid>eNp9kL1OwzAURj2ARCksPIFHhEixkyZxxqr8SlVhgNly7OvWVbAj26XA0-PQipHJ0udzv6t7ELqgZJJTwm6-w2ZC2aQsmyM0IjlrMkJIcYJOQ9gQQhkt6QjBvHPW2BV2Gsvb5QyDlU4NwYeJ0HVuBekbC6uwiQHDZ-8hBOMsTqkHhQMIvPVybew1fgmwVU6Cjd7FbcAKfultOEPHWnQBzg_vGL3d373OH7PF88PTfLbI2qIum0wzkALqtqy1mEJTVpSqWpU5A13rvNGtlJUqtGaqklpoaOtK5ppULRSSsFYVY3S175XeheBB896bd-G_OCV8kMKTFE4ZT1ISvNzDvQH_B65j7Plut-ND6mxnLPBNz6WzMd3FhY9GdsAHn3zwmdrIlBwKL_eFrXEujf2z-wdf9oSA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus</title><source>BioOne Open Access Titles</source><source>BioOne Complete</source><source>Open Access Titles of Japan</source><creator>Unuma, Tatsuya ; Okamoto, Hiroyuki ; Konishi, Kooichi ; Ohta, Hiromi ; Mori, Katsuyoshi</creator><creatorcontrib>Unuma, Tatsuya ; Okamoto, Hiroyuki ; Konishi, Kooichi ; Ohta, Hiromi ; Mori, Katsuyoshi</creatorcontrib><description>Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of game-togenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-length cDNA encoding vitellogenin (Vg; the MYP precursor in the coelomic fluid), and investigated its expression in various tissues of immature adults. The nucleotide sequence of Vg contains an open reading frame of 4050 bp encoding 1349 amino acids. The deduced amino acid sequence near the N-terminal showed 25% homology to the vertebrate transferrin family. Vitellogenin mRNA was detected in the ovary, testis, stomach, intestine and rectum by Northern blot analysis, with the highest level of mRNA expression in the gonad. Weak expression was also detected in the esophagus and coelomocytes by RT-PCR. In situ hybridization demonstrated that nutritive phagocytes, which exclusively fill the lumina of the immature gonad, contained Vg mRNA. These results suggested that the MYP stored in the immature gonads is synthesized and accumulated mainly within the nutritive phagocytes.</description><identifier>ISSN: 0289-0003</identifier><identifier>DOI: 10.2108/zsj.18.559</identifier><language>eng</language><publisher>Zoological Society of Japan</publisher><subject>Original s</subject><ispartof>Zoological Science, 2001-05, Vol.18 (4), p.559-565</ispartof><rights>2001 Zoological Society of Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b3759-f8ecae7b57fa4e95611d7d528ef7f29fbcc6d3ff8d6cfafeb76c2f06be3c08bd3</citedby><cites>FETCH-LOGICAL-b3759-f8ecae7b57fa4e95611d7d528ef7f29fbcc6d3ff8d6cfafeb76c2f06be3c08bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.2108/zsj.18.559$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>109,314,780,784,26978,27924,27925,52363,52719</link.rule.ids></links><search><creatorcontrib>Unuma, Tatsuya</creatorcontrib><creatorcontrib>Okamoto, Hiroyuki</creatorcontrib><creatorcontrib>Konishi, Kooichi</creatorcontrib><creatorcontrib>Ohta, Hiromi</creatorcontrib><creatorcontrib>Mori, Katsuyoshi</creatorcontrib><title>Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus</title><title>Zoological Science</title><description>Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of game-togenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-length cDNA encoding vitellogenin (Vg; the MYP precursor in the coelomic fluid), and investigated its expression in various tissues of immature adults. The nucleotide sequence of Vg contains an open reading frame of 4050 bp encoding 1349 amino acids. The deduced amino acid sequence near the N-terminal showed 25% homology to the vertebrate transferrin family. Vitellogenin mRNA was detected in the ovary, testis, stomach, intestine and rectum by Northern blot analysis, with the highest level of mRNA expression in the gonad. Weak expression was also detected in the esophagus and coelomocytes by RT-PCR. In situ hybridization demonstrated that nutritive phagocytes, which exclusively fill the lumina of the immature gonad, contained Vg mRNA. These results suggested that the MYP stored in the immature gonads is synthesized and accumulated mainly within the nutritive phagocytes.</description><subject>Original s</subject><issn>0289-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNp9kL1OwzAURj2ARCksPIFHhEixkyZxxqr8SlVhgNly7OvWVbAj26XA0-PQipHJ0udzv6t7ELqgZJJTwm6-w2ZC2aQsmyM0IjlrMkJIcYJOQ9gQQhkt6QjBvHPW2BV2Gsvb5QyDlU4NwYeJ0HVuBekbC6uwiQHDZ-8hBOMsTqkHhQMIvPVybew1fgmwVU6Cjd7FbcAKfultOEPHWnQBzg_vGL3d373OH7PF88PTfLbI2qIum0wzkALqtqy1mEJTVpSqWpU5A13rvNGtlJUqtGaqklpoaOtK5ppULRSSsFYVY3S175XeheBB896bd-G_OCV8kMKTFE4ZT1ISvNzDvQH_B65j7Plut-ND6mxnLPBNz6WzMd3FhY9GdsAHn3zwmdrIlBwKL_eFrXEujf2z-wdf9oSA</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Unuma, Tatsuya</creator><creator>Okamoto, Hiroyuki</creator><creator>Konishi, Kooichi</creator><creator>Ohta, Hiromi</creator><creator>Mori, Katsuyoshi</creator><general>Zoological Society of Japan</general><general>UniBio Press</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200105</creationdate><title>Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus</title><author>Unuma, Tatsuya ; Okamoto, Hiroyuki ; Konishi, Kooichi ; Ohta, Hiromi ; Mori, Katsuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b3759-f8ecae7b57fa4e95611d7d528ef7f29fbcc6d3ff8d6cfafeb76c2f06be3c08bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Original s</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Unuma, Tatsuya</creatorcontrib><creatorcontrib>Okamoto, Hiroyuki</creatorcontrib><creatorcontrib>Konishi, Kooichi</creatorcontrib><creatorcontrib>Ohta, Hiromi</creatorcontrib><creatorcontrib>Mori, Katsuyoshi</creatorcontrib><collection>CrossRef</collection><jtitle>Zoological Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Unuma, Tatsuya</au><au>Okamoto, Hiroyuki</au><au>Konishi, Kooichi</au><au>Ohta, Hiromi</au><au>Mori, Katsuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus</atitle><jtitle>Zoological Science</jtitle><date>2001-05</date><risdate>2001</risdate><volume>18</volume><issue>4</issue><spage>559</spage><epage>565</epage><pages>559-565</pages><issn>0289-0003</issn><abstract>Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of game-togenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-length cDNA encoding vitellogenin (Vg; the MYP precursor in the coelomic fluid), and investigated its expression in various tissues of immature adults. The nucleotide sequence of Vg contains an open reading frame of 4050 bp encoding 1349 amino acids. The deduced amino acid sequence near the N-terminal showed 25% homology to the vertebrate transferrin family. Vitellogenin mRNA was detected in the ovary, testis, stomach, intestine and rectum by Northern blot analysis, with the highest level of mRNA expression in the gonad. Weak expression was also detected in the esophagus and coelomocytes by RT-PCR. In situ hybridization demonstrated that nutritive phagocytes, which exclusively fill the lumina of the immature gonad, contained Vg mRNA. These results suggested that the MYP stored in the immature gonads is synthesized and accumulated mainly within the nutritive phagocytes.</abstract><pub>Zoological Society of Japan</pub><doi>10.2108/zsj.18.559</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0289-0003
ispartof Zoological Science, 2001-05, Vol.18 (4), p.559-565
issn 0289-0003
language eng
recordid cdi_crossref_primary_10_2108_zsj_18_559
source BioOne Open Access Titles; BioOne Complete; Open Access Titles of Japan
subjects Original s
title Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T04%3A01%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pier_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20cDNA%20encoding%20vitellogenin%20and%20its%20expression%20in%20red%20sea%20urchin,%20Pseudocentrotus%20depressus&rft.jtitle=Zoological%20Science&rft.au=Unuma,%20Tatsuya&rft.date=2001-05&rft.volume=18&rft.issue=4&rft.spage=559&rft.epage=565&rft.pages=559-565&rft.issn=0289-0003&rft_id=info:doi/10.2108/zsj.18.559&rft_dat=%3Cpier_cross%3Ehttp_www_pieronline_jp_content_article_0289_0003_18040_559%3C/pier_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true