Facile Preparation of PtNPs/BSA-RGO Nanostructure for Non-Enzymatic Glucose Electrochemical Sensing

A non-enzymatic glucose electrochemical sensing was constructed by platinum/bovine serum-reduced graphene (PtNPs/BSA-RGO) nanoconstructure modified glassy carbon electrode (GCE). The BSA-RGO composite was firstly synthesized using a hydrothermal synthesis method with BSA acted as the reductant and stabilizing reagent. Nanostructure Pt was reduced onto the BSA-RGO composites modified GCE with the formic acid reduction at room temperature. The BSA-RGO composites were characteried by Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) X-ray diffraction (XRD) and electrochemistry techniques, and the amperometric method was used to evaluate the electrocatalytic activity towards glucose in neutral media. Under optimal conditions, the PtNPs/BSA-RGO/GCE displays a linear range from 20 μM to 7.5 mM with a sensitivity of 3.62 mA mM-1 cm-2 (r = 0.9972), and a detection limit of 2.0 Μm (N/S=3) which lower than many non-enzymatic electrochemical glucose sensors. The modified electrode was applied to the determination of glucose in serum sample showed a satisfactory result, indicating it was promising for the development of a novel non-enzymatic electrochemical glucose sensor.