Rapid invitro Propagation of Bambusa balcooa Roxb. (Bamboo)

Production of bamboo plants by using vegetative propagation is not simple to establish a commercial fields however micropropagation techniques has much ability to achieve the bamboo seedling demands. In vitro cultures of Bambusa balcooa were achieved by using nodal segment as an explant. Eight different combinations of plant growth regulators with MS basal medium were used for shoot induction and multiplication. The medium with concentrations of BAP 1.5 mg/L + Kin 1.5 mg/L has gave maximum response for shoot induction (5.67) and also for shoot multiplication (13.67). For root induction five different IBA concentrations with MS basal medium ware evaluated. Maximum number of roots (6.34), highest root length (5.67) and more number of leaves (7.68) was obtained from MS basal medium containing 2.0 mg/L IBA. The well rooted in vitro regenerated plantlets of Bambusa balcooa are further hardened in soil: vermicompost: coco-peat as (1:1:1) proportion medium for primary hardening and riverbed sand: soil: farmyard manure as 1:1:1 proportion medium for secondary hardening. From the total hardened plantlets 90 % plantlets survived well after the secondary hardening stage.