Isolation, Identification and Molecular Characterization of Distinctive Isolates in Rhizosperic Soil of Coffee (Coffea arabica L.)

The current investigation was to identify and characterise the rhizospheric isolates in coffee leaves (Coffea arabica L.) that have many functions for stimulating plant growth. Coffee leaves were harvested from the plants growing at the Lower Pulney Hills Regional Coffee Research Station in Thandigudi. Morphological and biochemical characterization of seven different rhizospheric bacterial isolates was conducted. Recent studies have found varying degrees of resistance in rhizospheric bacterial isolates. Nitrogen fixation, phosphate solubilization, and potassium mobilization are all characteristics that help plants thrive. Additionally, the rhizospheric bacteria were prospective plant symbionts for granting biotic and abiotic stress tolerance. The three effective isolates were subjected to 16S rRNA and 18S rRNA sequencing, and the findings revealed that CPR-2 was Bacillus subtilis, CPR-4 was Pseudomonas fluorescens, and CPR-7 was Trichoderma harzianum and CPR-8 was Rhizoctonia solani.