Invitro Efficacy Testing of Bacteriocin against Multidrug Resistant Pseudomonas aeruginosa

Bacteriocin is a protein substance possessing antimicrobial activities during the growth by great number of Gram positive and Gram negative bacteria (Zacharof and Lovitt). It will destabilize the cytoplasmic membrane and kills bacteria. Various studies reported antibacterial activity of bacteriocin against Listeria and other Gram positive bacteria. Hence, there is a need to know the effectiveness of bacteriocin against drug resistant Gram negative bacteria especially Pseudomonas aeruginosa, the leading cause of Hospital Acquired Infection (HAI). Objective of the research was to find the efficacy testing of bacteriocin against multidrug resistant Pseudomonas aeruginosa, to isolate and identify multidrug resistant Pseudomonas aeruginosa. To extract and purify bacteriocin from lactobacillus acidophilus and to perform invitro efficacy testing of bacteriocin against multidrug resistant Pseudomonas aeruginosa. Conventional identification and antimicrobial susceptibility was performed based on conventional procedure. The study was done at the clinical microbiology laboratory of Saveetha Medical College and hospital during the period of January to June 2020. Total 60 samples were collected from both male and female and from all the age groups. Among that 60 samples 39% were woundswab, 29% were urine, 5% were pus, 3% were tissue culture, 3% of samples were tracheal culture. The strains of Pseudomonas aeruginosa collected for this study shows resistance to cefepime, ceftazidime, ciprofloxacin, meropenem, imipenem, gentamicin, cotrimaxazole and piperacillin-tazobactam. Bacteriocins were protein in nature and simple proteins to particles resembling components of bacteriophage. The zone of inhibition formation is strong in vitro and can act against the pathogenesis of Pseudomonas aeruginosa. Disinfectants have ineffective in controlling the nosocomial infection in the spread of pseudomonas aeruginosa. Bacteriocins not only can support the disinfectants, it can also be combined with antibiotics when purified and processed.